Development of an electrochemical-surface enhanced Raman spectroscopic (EC-SERS) sensor for bacterial screening

Show simple item record

dc.contributor.advisor Brosseau, Christa L.
dc.contributor.advisor Sit, Clarissa
dc.creator Lynk, Taylor Paige
dc.date.accessioned 2019-06-13T14:49:19Z
dc.date.available 2019-06-13T14:49:19Z
dc.date.issued 2018
dc.identifier.uri http://library2.smu.ca/handle/01/28923
dc.description.abstract The lack of efficiency of current methods for bacterial screening has prompted an increasing interest in developing a cost-effective, rapid and sensitive alternative for applications in all sectors of society. This project aims to explore a new detection platform for bacterial screening by coupling an applied electric potential with surface-enhanced Raman spectroscopy (SERS), a technique termed electrochemical surface-enhanced Raman spectroscopy (EC-SERS). The goal of using this technique is to improve upon the sensitivity and reproducibility of normal SERS to allow for rapid, point-of-need (PON) bacterial detection and identification. This project first shows the EC-SERS characterization of the commonly observed nucleotide breakdown products that dominate the SERS spectra of bacteria: adenine, guanine, xanthine, hypoxanthine, uric acid, 5’-adenosine monophosphate (AMP), and guanosine. This thesis work then concentrates on developing a sample preparation method to be used to study bacteria using EC-SERS for the first time. The results of this project demonstrate the improvement of the SERS spectra of both E. coli K-12 and B. megaterium bacteria obtained when an electric potential is employed, and highlights the great promise of EC-SERS for use as a fast and affordable bacterial screening method. en_CA
dc.language.iso en en_CA
dc.publisher Halifax, N.S. : Saint Mary's University
dc.title Development of an electrochemical-surface enhanced Raman spectroscopic (EC-SERS) sensor for bacterial screening en_CA
dc.type Text en_CA
thesis.degree.name Bachelor of Science (Honours Chemistry)
thesis.degree.level Undergraduate
thesis.degree.discipline Chemistry
thesis.degree.grantor Saint Mary's University (Halifax, N.S.)


Files in this item

 
 

This item appears in the following Collection(s)

Show simple item record