The determination of nitroimidazole residues in fish and fish products

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dc.contributor.advisor MacNeil, James D.
dc.contributor.advisor Lamoureux, Marc Maurice, 1961-
dc.creator Watson, Lynn 2012-11-08T15:22:25Z 2012-11-08T15:22:25Z 2011
dc.identifier.other SH336 A5 W38 2011
dc.description xvii, 278 leaves : ill. ; 29 cm. en_CA
dc.description Includes abstract.
dc.description Includes bibliographical references (leaves 156-164).
dc.description.abstract A method was developed and validated for the determination of nitroimidazoles (NIs) including 2-hydroxymethyl-1-methyl-5-nitroimidazole (HMMNI), ipronidazole (IPZ), 1-methyl-2-(2'-hydroxyisopropyl)-5-nitroimidazole (IPZ-OH), metronidazole (MNZ), 1-(2-hydroxyethyl)-2-hydroxymethyl-5-nitroimidazole (MNZ-OH), ronidazole (RNZ) and dimetridazole (DMZ) in fish and crustaceans by ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). The compounds were extracted with acidic acetonitrile, followed by solid phase extraction (SPE) C18 clean up and hexane washing. The validated method has a linear range from 0-50 ng/g in target representative species (tilapia, salmon and shrimp), with LOD from 0.07 - 1.0 ng/g and LOQ from 0.21 - 3.0 ng/g, depending on the analyte. Recoveries ranged from 87 to 121% for the analytes of interest. Method precision ranged from 6 to 26% RSD with HorRat values within typical limits of acceptability. The method successfully analyzed rainbow trout samples treated with MNZ in a depletion study under controlled conditions and is suitable for use in a regulatory monitoring program for residues of nitroimidazoles in seafood and aquacultured products. en_CA
dc.description.provenance Submitted by Dianne MacPhee ( on 2012-11-08T15:22:25Z No. of bitstreams: 0 en
dc.description.provenance Made available in DSpace on 2012-11-08T15:22:25Z (GMT). No. of bitstreams: 0 Previous issue date: 2011 en
dc.language.iso en en_CA
dc.publisher Halifax, N.S. : Saint Mary's University en_CA
dc.subject.lcc SH336 A5 W38 2011
dc.subject.lcsh Fishery products -- Testing
dc.subject.lcsh Seafood -- Testing
dc.subject.lcsh Antibiotic residues
dc.subject.lcsh Nitroimidazoles
dc.title The determination of nitroimidazole residues in fish and fish products en_CA
dc.type Text en_CA Master of Science in Applied Science Masters Chemistry Saint Mary's University (Halifax, N.S.)
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